Journal:

Article Title: Using a Resequencing Microarray as a Multiple Respiratory Pathogen Detection Assay ▿ †

doi: 10.1128/JCM.01870-06

Figure Lengend Snippet: Analytic sensitivity of microarray-based detection for prototype control strains

Article Snippet: Thus, hybridization to a series of probe sets provides redundant presence/absence information for the organism while also revealing strain-specific single-nucleotide polymorphisms relative to the sequence chosen. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Organism a Sample type a Strain Sample source b Detection limit (genome copies) c HAdV-4 d DNA RI-67 ATCC 10 2 HAdV-4 vaccine DNA CL68578 NHRC 10 2 HAdV-4FS_Navy DNA NRHC 10 2 HAdV-4FS_AirForce DNA AFIOH 10 2 HAdV-4FS_AirForce Viral particles ADL 10 2 HAdV-5 DNA Adenoid 75 ATCC 10 3 HAdV-7 DNA Gomen ATCC 10 2 HAdV-7 Viral particles Gomen ATCC ND HAdV-7a vaccine DNA 55142 NHRC 10 2 HAdV-7FS_Navy DNA NHRC 10 3 B. anthracis DNA Ames AFIP 10 1 B. anthracis Bacterial cells Sterne CRP ND B. pertussis DNA NHRC 10 2 Chlamydia pneumoniae DNA ABi 10 1 Influenza A virus (H1N1) Viral particles PR/8/34 ABi 10 2 Influenza A virus (H3N2) RNA ADL 10 2 Influenza A virus (H5N1) RNA AFIOH 10 1 Influenza B virus Viral particles B/Lee/40 ABi 10 3 Francisella tularensis DNA SCHU4 ATCC 10 3 F. tularensis Bacterial cells SCHU4 CRP ND Human coronavirus DNA/RNA 229E ATCC 10 3 Human coronavirus Viral particles 229E ATCC ND Human coronavirus DNA/RNA OC43 ATCC 10 3 Human coronavirus Viral particles OC43 ATCC ND Rhinovirus 89 Viral particles 41467 Gallo ATCC 10 3 Lassa virus e Plasmids BlueHeron 10 3 M. pneumoniae DNA AFIP 10 3 M. pneumoniae Bacterial cells NHRC ND N. meningitidis DNA Murray ATCC 10 2 Parainfluenza virus 1 Viral particles C-35 ATCC 10 3 Parainfluenza virus 3 Viral particles C 243 ATCC 10 3 RSV A Viral particles A-2 ATCC 10 3 RSV B Viral particles B WV/14617/85 ATCC 10 2 S. pneumoniae DNA AFIP 10 2 S. pyogenes DNA Rosenbach ATCC 10 3 S. pyogenes Bacterial cells NHRC ND Variola major virus e Plasmids BlueHeron 10 3 Vaccinia virus DNA Lister ABi 10 3 Y. pestis DNA D27 AFIP 10 3 Ebola virus e Plasmids BlueHeron 10 3 Open in a separate window a Samples were generated by mixing purified nucleic acid templates in Tris-EDTA buffer to create 10 6 genome copies/μl of stock solution.

Techniques: Microarray, Control, Virus

Journal:

Article Title: Using a Resequencing Microarray as a Multiple Respiratory Pathogen Detection Assay ▿ †

doi: 10.1128/JCM.01870-06

Figure Lengend Snippet: Differentiation by RPM v.1 of various HAdVs causing febrile respiratory infections

Article Snippet: Thus, hybridization to a series of probe sets provides redundant presence/absence information for the organism while also revealing strain-specific single-nucleotide polymorphisms relative to the sequence chosen. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Organism a Sample type a Strain Sample source b Detection limit (genome copies) c HAdV-4 d DNA RI-67 ATCC 10 2 HAdV-4 vaccine DNA CL68578 NHRC 10 2 HAdV-4FS_Navy DNA NRHC 10 2 HAdV-4FS_AirForce DNA AFIOH 10 2 HAdV-4FS_AirForce Viral particles ADL 10 2 HAdV-5 DNA Adenoid 75 ATCC 10 3 HAdV-7 DNA Gomen ATCC 10 2 HAdV-7 Viral particles Gomen ATCC ND HAdV-7a vaccine DNA 55142 NHRC 10 2 HAdV-7FS_Navy DNA NHRC 10 3 B. anthracis DNA Ames AFIP 10 1 B. anthracis Bacterial cells Sterne CRP ND B. pertussis DNA NHRC 10 2 Chlamydia pneumoniae DNA ABi 10 1 Influenza A virus (H1N1) Viral particles PR/8/34 ABi 10 2 Influenza A virus (H3N2) RNA ADL 10 2 Influenza A virus (H5N1) RNA AFIOH 10 1 Influenza B virus Viral particles B/Lee/40 ABi 10 3 Francisella tularensis DNA SCHU4 ATCC 10 3 F. tularensis Bacterial cells SCHU4 CRP ND Human coronavirus DNA/RNA 229E ATCC 10 3 Human coronavirus Viral particles 229E ATCC ND Human coronavirus DNA/RNA OC43 ATCC 10 3 Human coronavirus Viral particles OC43 ATCC ND Rhinovirus 89 Viral particles 41467 Gallo ATCC 10 3 Lassa virus e Plasmids BlueHeron 10 3 M. pneumoniae DNA AFIP 10 3 M. pneumoniae Bacterial cells NHRC ND N. meningitidis DNA Murray ATCC 10 2 Parainfluenza virus 1 Viral particles C-35 ATCC 10 3 Parainfluenza virus 3 Viral particles C 243 ATCC 10 3 RSV A Viral particles A-2 ATCC 10 3 RSV B Viral particles B WV/14617/85 ATCC 10 2 S. pneumoniae DNA AFIP 10 2 S. pyogenes DNA Rosenbach ATCC 10 3 S. pyogenes Bacterial cells NHRC ND Variola major virus e Plasmids BlueHeron 10 3 Vaccinia virus DNA Lister ABi 10 3 Y. pestis DNA D27 AFIP 10 3 Ebola virus e Plasmids BlueHeron 10 3 Open in a separate window a Samples were generated by mixing purified nucleic acid templates in Tris-EDTA buffer to create 10 6 genome copies/μl of stock solution.

Techniques: